P203 Phenotypic characterization and redifferentiation of human articular chondrocytes expanded on microcarriers
نویسندگان
چکیده
منابع مشابه
Prostaglandins Modulate the Redifferentiation of Monolayer-expanded Adult Human Articular Chondrocytes
Introduction. Adult human articular chondrocytes (AHAC) dedifferentiated by monolayer expansion are known to have a limited ability to re-enter the differentiation program and generate cartilage tissue ex vivo. We recently reported that specific growth factors during the expansion and redifferentiation phases of AHAC culture enhance in vitro chondrogenesis (1). Using that system, we now investi...
متن کاملDerivation, characterization and expansion of fetal chondrocytes on different microcarriers.
Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon microcarriers and...
متن کاملPhenotypic modulation of human articular chondrocytes by bistratene A.
Chondrocytes undergo phenotypic alterations following extended periods in monolayer culture, i.e., they become bipolar and flattened, proliferate, and synthesise type I as opposed to type II collagen. This process has been termed chondrocyte dedifferentiation. Bistratene A is a macrolide polyether that specifically activates the delta isoform of protein kinase C (PKCdelta) in some cell types. H...
متن کاملEvaluation of CD98 Expression in Normal and Osteoarthritic Human Articular Chondrocytes
Background: Recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. Integrin signaling may be regulated by a number of mechanisms including accessory proteins such as CD98 (4F2 antigen). Objectives: To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechano...
متن کاملSurface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes
Autologous chondrocyte implantation (ACI) is a method of cartilage repair. To improve the quality of regenerated tissue by ACI, it is essential to identify surface marker expression correlated with the differentiation status of monolayer expanded human articular chondrocytes and to define the index for discriminating dedifferentiated cells from monolayer expanded human articular chondrocytes. N...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2007
ISSN: 1063-4584
DOI: 10.1016/s1063-4584(07)61558-1